Oligonucleotide Synthesis

Oligonucleotide or oligo synthesis is greatly beneficial for scientific research. It is one of the most important techniques nowadays in laboratory practices.

Has great importance!

It offers an inexpensive and rapid access to personalized oligonucleotides. The design of primer Oligonucleotide synthesis is a fundamental step for the success of methods based on the DNA polymerase chain reaction (PCR).

Process needs purification

In the production of oligonucleotides, in addition to complete oligo, some impurities are also generated. These are residues and by-products of the reactions involved in the oligo synthesis. Therefore, the type of purification to be chosen is important to ensure the degree of purity that your research needs –

·         Desalination –is composed of a porous gel in which the particles are separated.

·         RP-OPC – consists of a column filled with high performance polymeric resin.

·         HPLC – gradually removing impurities according to how hydrophobic they are.

Phosphoramidite methodology

The synthesis of oligonucleotides takes place through a methodology of adding Phosphoramidite in a solid synthesis support. The synthetic cycle consists of 4 phases – Coupling, Block, Oxidation, and Detritilation.